Immobilization Buffer (pH 4.0 - 6.0)

Immobilization buffers are used to dilute the ligand for direct coupling onto the sensor surface of a Carboxyl Cartridge. The low pH and salt content is ideal for pre-concentration of the ligand to the Carboxyl sensor surface, increasing immobilization efficiency. It is recommended to find the optimal pH for a ligand experimentally, but typically a pH that is 0.5 units below the pI of the ligand can be used. Each immobilization buffer is ready-to-use in Alto cartridges, sufficient for up to 10 experiments to be used with the Alto Carboxyl Surfacing Kit.

Available immobilization buffers include:

• 10 mM Sodium Acetate, pH 4.0 (ALTO-R-IMB-4.0)
• 10 mM Sodium Acetate, pH 4.5 (ALTO-R-IMB-4.5)
• 10 mM Sodium Acetate, pH 5.0 (ALTO-R-IMB-5.0)
• 10 mM Sodium Acetate, pH 5.5 (ALTO-R-IMB-5.5)
• 10 mM MES, pH 6.0 (ALTO-R-IMB-6.0)

Additional information

Pricing is valid in North America only. For international pricing, please contact your local Distributor.